2/18/2011
Modulation of collagen and keratin synthesis in co-cultures of fibroblasts and keratinocytes on hyaluronan-coated polysulfone membranes
(71-88) Journal of Bioactive and Compatible Polymers 26 #1 (2011)
Abstract
Attia et al of the University of Caen  and University of Rouen, France co-cultured human epidermal keratinocytes and dermal fibroblasts on polysulfone (PSU) membranes, previously coated or not with hyaluronan (HA), and compared to monocultured keratinocytes and fibroblasts. The co-cultures reduced types I and III collagen levels, indicating that keratinocytes exerted an inhibition on matrix synthesis by fibroblasts. On the other hand, the amounts of keratins 17 and 10 were increased, suggesting that fibroblasts stimulate the production of keratins by keratinocytes. In contrast with naked PSU membranes, HA coatings increased types I and III collagens mRNA (messenger ribonucleic acid) levels, suggesting that HA counteracts the inhibition produced by keratinocytes.  Changes were also observed in the expression of metalloproteinases (MMPs) on HA-coated PSU membranes. The presence of keratinocytes increased MMP1 and MMP3 synthesis by fibroblasts whereas HA exerted an inhibitory effect on MMP2 expression that depended on the culture conditions.  (RDC 2/19/2011)